Fast detection of auxins by microplate technique

Abstract

" Plant growth promotion Indole-3-acetic acid (IAA) is the most abundant natural auxin that plays diverse roles in plant growth, development and plant immunity. Perturbing auxin homeostasis appears to be a common virulence mechanism, as many pathogens can synthesize auxin-like molecules (Spoel and Dong, 2008). In other hand, the addition of plant growth promotion rhizobacterias (PGPR) that are able to produce auxins promote plant growth and provide protection against pathogens. Techniques as HPLC and GC are used to quantify auxins produced by microorganism and plants at high precision and sensitivity, eventhough those technique are expensive and requires big amount of solvents. For these reason, the aim of the present study was developed a fast microplate technique for auxin detection, in Bacillus subtilis strains using salkowski reagent. For auxin quantification were done calibration curves with alcohol, landy medium and water and the R2 were calculated. The microplate techniques were able to quantify auxin production by B. subtillis stains."